ABSTRACT
Em março de 2012 foi diagnosticado um surto de doença reprodutiva em rebanho bovino no Estado da Paraíba, Brasil. Foram examinadas 32 vacas e dois touros da raça Girolando. As vacas apresentaram sinais de doença reprodutiva como repetição de cio, vulvovaginite granular, infertilidade e abortos. As amostras de suabes vaginais e prepuciais foram colhidas e submetidas a isolamento bacteriano e PCR. As reações da PCR para Mollicutes e Ureaplasma spp. foram realizadas com os iniciadores MGSO-GPO3 e UGP'F-UGP'R, respectivamente. Na Nested PCR para Ureaplasma diversum, os iniciadores usados foram UD1, UD2, UD3 e UD4. Para isolamento bacteriano, as amostras foram diluídas de 10-1 até 10-5, semeadas em meio "UB", líquido e placa, sendo incubadas por até 21 dias a 37ºC em jarra de microaerofilia. A frequência de Mollicutes detectada na PCR foi de 65,6% e para Ureaplasma spp. foi de 50,0%, enquanto que para U. diversum foi de 15,6%. No isolamento a frequência de Mollicutes foi de 57,1% e para Ureaplasma spp. foi de 28,6%. No ágar "UB" foi visualizado o crescimento misto de Mycoplasma spp. e Ureaplasma spp. em seis amostras. Foi confirmado o envolvimento de micro-organismos da Classe Mollicutes em surto de doença reprodutiva em vacas no sertão paraibano.
In March of 2012 was investigated a reproductive disease outbreak in cattle herds from Paraíba State, Brazil. Were examined 32 cows and two bulls Giroland breed. The cows showed signs and symptoms of reproductive failure such as repeat breeding, granular vulvovaginitis, infertility and abortions. Vaginal and preputial mucous samples were collected for analysis by PCR and isolation. The PCR reactions for Mollicutes and Ureaplasma spp. were realized with primers MGSO and GPO3, and UGP'F and UGP'R respectively. The nested PCR assay for Ureaplasma diversum was realized with primers UD1, UD2, UD3 and UD4. For bacteriologic isolation, obtained samples were diluted up to 10-1 at 10-5, inoculated into liquid and solid "UB" medium, and incubated for up to 21 days, at 37ºC in microaerophilie jar. In the PCR reactions the frequency of Mollicutes detected in the analyzed vaginal mucous samples was 65.6, for Ureaplasma spp. was 50.0, while for U. diversum was 15.6. The frequency for isolation of Mollicutes was of 57.1 and for Ureaplasma spp. was of 28.6. In the UB agar was visualized growth of Mycoplasma spp. and Ureaplasma spp., associated in six of the samples. In the cows the presence of Mollicutes and Ureaplasma spp. was confirmed for the reproductive disease outbreak in the semiarid region of Paraiba.
Subject(s)
Animals , Female , Cattle , Ureaplasma Infections/veterinary , Tenericutes/isolation & purification , Ureaplasma/isolation & purification , Abortion, Veterinary , Genital Diseases, Female/veterinary , Infertility/veterinary , Vulvovaginitis/veterinaryABSTRACT
Infectious pustular vulvo vaginitis [IPV] was isolated and identified from cows showing the genital form of infection, located at Dakahlia Governorate. The clinical viral sign included vulvo-vaginitis, abortion, retained fetal membranes and metritis. IPV was isolated from vaginal swabs on MDBK cell line and confirmed by virus neutralization test [VNT]. SNT and ELIZA detected IBR/IPV antibodies in serum and milk samples. SNT and ELISA revealed that 63 [70%] and 71 [78.1%] sera samples were positive respectively while 35 [58.3%] and 40 [66.7%] milk samples were positive respectively. The biochemical changes are more pronounced associated with sera containing high IBR/IPV antibody titer. There were significant increase protein bands with molecular weight 193.87, 163.97, 156.81, 129.70, 105.03, 100.94, 87.74, 74.67, 73.96 KD and significant decrease of protein band with molecular weight [KD] 138.69, 68.55, 66.63, 65.09, 41.36 and 30.39 KD in sera with low, moderate and high IBR/IPV antibody titer associated with several mineral changes including increase magnesium and iron protein binding capacity associated with serum with low IBR/IPV antibodies titer, while moderate IBR/IPV antibodies sera associated with significant increase in total calcium, inorganic phosphorus, total magnesium, iron and iron binding capacity levels and significant decrease in chloride levels, while significant increase in inorganic phosphorus, magnesium, iron and iron binding capacity levels and significant decrease in chloride level ions was determined in serum with high IBR/IPV antibody titer. The increase in IBR/IPV antibody concentration was associated with metabolic changes that lead to an increased individual animal risk of non-pregnancy and increased risk of abortion. All animals imported to Egypt should be free from IBR/IPV infections. IBR/IPV virus as cause of venereal disease must consider. Control programs for detection and removal of IBR/IPV-persisted cattle should be applied in cattle herds all over the country
Subject(s)
Animals , Milk/microbiology , Vulvovaginitis/veterinary , Herpesvirus 1, Bovine/isolation & purification , Serology/methods , Cattle/immunology , Enzyme-Linked Immunosorbent Assay/methodsABSTRACT
A detecçäo de anticorpos anti-Herpesvírus Bovino tipo 1 (BHV-1) foi realizada, através da técnica de soroneutralizaçäo, em 1235 amostras de soro de bovinos adultos, näo vacinados contra Rinotraqueíte Infecciosa Bovina. As amostras de soro analisadas foram colhidas em 81 rebanhos, com histórico de problemas reprodutivos, incluindo animais com aptidäo para carne e leite, provenientes de 30 municípios do Estado do Paraná. Na amostragem proveniente de rebanhos leiteiros, 41,9 por cento (409/977) das amostras de soro e 90,5 por cento (57/63) dos rebanhos foram considerados positivos. Em bovinos de corte, o índice de soropositividade foi de 50,8 por cento (131/258) e 100 por cento (18/18) para amostras de soro e rebanhos, respectivamente. As frequências de 43,7 por cento (540/1235) de animais e 92,6 por cento (75/81) de rebanhos soropositivos demonstram que as infecçöes por BHV-1 apresentam-se amplamente disseminadas nas regiöes estudadas.